Journal: Heliyon

Article Title: BushenHuoxue decoction suppresses M1 macrophage polarization and prevents LPS induced inflammatory bone loss by activating AMPK pathway

doi: 10.1016/j.heliyon.2023.e15583

Figure Lengend Snippet: Activation of AMPK signaling pathway by BSHXD. (A) P -AMPKα1 and AMPKα1 protein levels at various times, detected by Western blot analysis. (B) The relative levels of P - AMPKα1/AMPKα1. (C) PP2Aα+β, p -AMPKα1 and AMPKα1 protein levels, detected by Western blot analysis. The relative levels of (D) PP2Aα+β/β-Actin, (E)AMPKα1/β-Actin and (F) P - AMPKα1/AMPKα1. N = 3. Data are presented as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 compared to LPS group.

Article Snippet: The following antibodies were used: IL-1β(#31202, CST), IL-6 (ab259341, Abcam), TNF-α (ab183218, Abcam), CD86 (A1199, Abclonal), AMPKα1 (A1229, Abclonal), P -AMPKα1 (#4184, CST), PP2Aα+β (ab168350, Abcam) and β-Actin (#12262,CST), Goat Anti-Mouse (#7074, CST) and Anti-rabbit (#91196, CST).

Techniques: Activation Assay, Western Blot

Journal: Nutrients

Article Title: Anti-Obesity Effects of Aqueous Extracts of Sunbanghwalmyung-Eum in High-Fat- and High-Cholesterol-Diet-Induced Obese C57BL/6J Mice

doi: 10.3390/nu14142929

Figure Lengend Snippet: Primary and secondary antibodies used for Western blotting.

Article Snippet: AMPKα , 1:1000 , sc-5298 , Santa , Goat-anti-rabbit , 1:5000 , ADI-SAB-300 , Enzo.

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Identification of an Annonaceous Acetogenin Mimetic, AA005, as an AMPK Activator and Autophagy Inducer in Colon Cancer Cells

doi: 10.1371/journal.pone.0047049

Figure Lengend Snippet: (A) Immunoblot analysis of LC3-I and LC3-II levels in LOVO cells treated with AA005. (B) LOVO cells transfected with pQCXIP-GFP or pQCXIP-GFP-LC3 plasmid were treated for 24 h with AA005 (100 nM) and/or inosine (20 mM), and rapamycin (100 nM), and assessed by immunofluorescence analyses. (C) LOVO cells were treated with indicated protocols, lysed, and Western blot assay was performed using indicated antibodies. (D) LOVO cells were treated with AA005 or cisplatin (20 µM) for 24 h, or AA005 at 100 nM for indicated time points. The cells were then analyzed by Annexin V/PI staining and flow cytometry. (E) Western blot analyses of lysates of LOVO cells treated with AA005 or cisplatin (20 µM for 24 h).

Article Snippet: The antibodies used in this study were as follows: anti-β-Actin (Sigma); anti-p-AMPKα1, anti-p-ACC, anti-p-mTOR, anti-p-S6K, anti-AMPKα1, anti-ACC, anti-mTOR, anti-S6K, anti-PARP, goat anti-rabbit IgG-HRP and goat anti-mouse IgG-HRP antibody (Cell Signaling Technology); anti-CyclinD1 (Abcam), anti-CDK4 (Santa Cruz Biotechnology), and anti-LC3 (Sigma).

Techniques: Western Blot, Transfection, Plasmid Preparation, Immunofluorescence, Staining, Flow Cytometry